HDAC developer (2X)

2x HDAC Assay Developer is a reagent designed to be used with BPS Bioscience HDAC (histone deacetylase) or sirtuin fluorogenic assay kits containing Fluorogenic HDAC/SIRT Substrate 1 (BPS Bioscience #50032), Fluorogenic HDAC Class 2a Substrate (BPS Bioscience #50040), Fluorogenic HDAC Class 2a Substrate 2 (Green) (BPS Bioscience #50042), Fluorogenic HDAC Substrate 2 (Green) (BPS Bioscience #50038) and Fluorogenic HDAC Substrate 3 (BPS Bioscience #50037). Use of an appropriate fluorogenic substrate, containing acetylated lysine side chain, and incubation with a sample containing HDAC activity (purified enzyme) results in deacetylation of the substrate. This deacetylation of the substrate allows the generation of a fluorescent signal when incubated with HDAC Assay Developer. Trichostatin A is included in the formulation to stop Class 1 and 2 HDACs reactions and allow measurement of HDAC activity at the time of addition.

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