HDAC Fluorogenic Assay Kit (Green)

The Fluorogenic HDAC Assay Kit (Green) is a complete assay system designed to measure histone deacetylase (HDAC) class 1 activity for screening and profiling applications. The kit includes a specific HDAC substrate that emits light within the green light spectrum (ex 485 nm/em 528 nm)*.

*Note: This kit is particularly suitable if the researcher’s test inhibitor sample(s) fluoresce in the same range as the substrates in our other HDAC assay kits, ex 350-380 nm/em 440-460 nm. If inhibitor fluorescence is not an issue, the Fluorogenic HDAC Assay Kit, catalog #50033 is recommended.

The Fluorogenic HDAC Assay Kit (Green) comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC activity measurements. In addition, the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC Assay Kit (Green) is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader at 485 nm (excitation)/528 nm (emission).

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